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C1q / tumor necrosis factor-alpha-related proteins (CTRPs)

C1q / tumor necrosis factor-alpha-related proteins (CTRPs)

Adipose tissue functions as storage of energy and plays an active role in monitoring and controlling whole-body metabolism by secreting a number of proinflammatory adipokines, (such as leptin, resistin, tumor necrosis factor-alpha and interleukin-6), as well as one anti-inflammatory adipokine, adiponectin. Adiponectin is involved in controlling the whole-body metabolism, particularly by increasing insulin sensitivity in muscle and liver, and by increasing fatty acid oxidation in muscle (1). Recently, a new highly conserved family of adiponectin paralogs designated as C1q/Tumor Necrosis Factor-alpha-Related Proteins (CTRPs) 1-7, has been described in mouse and human (2-5). This family of proteins exhibits similar structural and biological properties to adiponectin. The CTRPs, unlike Adiponectin whose expression is restricted to adipose tissue, are widely expressed by many different adult mouse tissues. One member, the mouse CTRP2 (mCTRP2) is the closest paralog of adiponectin. CTRP2 (mouse), like adiponectin, rapidly induces phosphorylation of AMP-activated protein kinase, acetyl-CoA carboxylase, and mitogen-activated protein kinase in C2C12 myotubes, which resulted in increased glycogen accumulation and fatty acid oxidation. The activity of mCTRP2 suggests that some members of the CTRP family of proteins could substitute for adiponectin function in vivo.

CTRP7 Detection Set (APO-54N-023)

Summary of features

	For the quantitative determination of human and mouse CTRP7 from biological fluids (serum and cell culture supernatant)
	Monoclonal antibodies-based sandwich ELISA
	Detection limit: 0.1 ng / ml
	Range: 0-10 ng / ml
	Specificity : Detects human and mouse CTRP7
	Format: sufficient materials to run ELISAs on 5 x 96-well plates

Principle of the Kit

This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA) developed for the direct measurement of mouse and human CTRP7 (CTRP7) in biological fluids. A monoclonal antibody (AB) specific for CTRP7 (COAT) is coated onto the wells of the microtiter plate. Samples and standards of CTRP7 are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, CTRP7 is recognized by the addition of a biotinylated monoclonal antibody specific for CTRP7 (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of CTRP7 in the samples.

Figure : Linearity of the STD curve.

LITERATURE OVERVIEW:

1. Fantuzzi, G. Adipose tissue, adipokines, and inflammation. J Allergy Clin Immunol 115, 911-9; quiz 920 (2005).

2. Wong, G. W., Wang, J., Hug, C., Tsao, T. S. & Lodish, H. F. A family of Acrp30/adiponectin structural and functional paralogs. Proc Natl Acad Sci U S A 101, 10302-7 (2004).

3. Lasser, G. et al. C1q-TNF related protein-1 (CTRP-1), a vascular wall protein that inhibits collagen-induced platelet aggregation by blocking vWF binding to collagen. Blood (2005).

4. Maeda, T. et al. Cartducin, a paralog of Acrp30/adiponectin, is induced during chondrogenic differentiation and promotes proliferation of chondrogenic precursors and chondrocytes. J Cell Physiol (2005).

5. Weigert, J. et al. The adiponectin paralog CORS-26 has anti-inflammatory properties and is produced by human monocytic cells. FEBS Lett (2005).